Quick Search: Journal  Author  Title/Abstract  Vol./Year 

Appl. Phys. Express 4 (2011) 026201 (2 pages)  |Previous Article| |Next Article|  |Table of Contents|
|Full Text PDF (405K)| |Buy This Article|

Plasma-Assisted Biological Macromolecular Crystallization

Tsuyohito Ito1,4, Takuhiro Ito2,3,4 , and Shigeyuki Yokoyama2,3,4

1Frontier Research Base for Global Young Researchers, Frontier Research Center, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan
2Laboratory of Structural Biology, Graduate School of Science, The University of Tokyo, Bunkyo, Tokyo 113-0033, Japan
3Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, Bunkyo, Tokyo 113-0033, Japan
4RIKEN Systems and Structural Biology Center, Tsurumi, Yokohama 230-0045, Japan

(Received January 5, 2011; accepted January 19, 2011; published online February 8, 2011)

Improvement in the crystallization probability of biological macromolecules by plasma irradiation is demonstrated with lysozymes from hen egg whites. This novel technique of “plasma-assisted biological macromolecular crystallization” is readily carried out with a relatively simple apparatus, and is likely to become a powerful tool in discerning the three-dimensional structure of such macromolecules. ©2011 The Japan Society of Applied Physics

URL: http://apex.jsap.jp/link?APEX/4/026201/
DOI: 10.1143/APEX.4.026201
PACS: 52.80.Wq, 81.10.Dn, 87.15.nt, 87.14.ef


|Full Text PDF (405K)| |Buy This Article| Citation:

References

  1. L. J. DeLucas, C. D. Smith, H. W. Smith, S. Vijay-Kumar, S. E. Senadhi, S. E. Ealick, D. C. Carter, R. S. Snyder, P. C. Weber, F. R. Salemme, D. H. Ohlendorf, H. M. Einspahr, L. L. Clancy, M. A. Navia, B. M. McKeever, T. L. Nagabhushan, G. Nelson, A. Mcpherson, S. Koszelak, G. Taylor, D. Stammers, K. Powell, G. Darby, and C. E. Bugg: Science 246 (1989) 651
  2. H. Adachi, K. Takano, Y. Hosokawa, T. Inoue, Y. Mori, H. Matsumura, M. Yoshimura, Y. Tsunaka, M. Morikawa, S. Kanaya, H. Masuhara, Y. Kai, and T. Sasaki: Jpn. J. Appl. Phys. 42 (2003) L798[JSAP].
  3. M. Teschke, J. Kedzierski, E. G. Finantu-Dinu, D. Korzec, and J. Engemann: IEEE Trans. Plasma Sci. 33 (2005) 310.
  4. B. L. Sands, B. N. Ganguly, and K. Tachibana: Appl. Phys. Lett. 92 (2008) 151503[AIP Scitation].
  5. J. Shi, F. Zhong, J. Zhang, D. W. Liu, and M. G. Kong: Phys. Plasmas 15 (2008) 013504.
  6. K. Urabe, Y. Ito, K. Tachibana, and B. N. Ganguly: Appl. Phys. Express 1 (2008) 066004[JSAP].
  7. E. Wagenaars, M. D. Bowden, and G. M. W. Kroesen: Phys. Rev. Lett. 98 (2007) 075002[APS].
  8. A. Kaminska, H. Kaczmarek, and J. Kowalonek: Eur. Polym. J. 38 (2007) 1915.
  9. I. Yoshizaki, T. Sato, N. Igarashi, M. Natsuisaka, N. Tanaka, H. Komatsu, and S. Yoda: Acta Crystallogr., Sect. D 57 (2001) 1621.
|TOP|  |Previous Article| |Next Article|  |Table of Contents| |APEX Home|
Copyright © 2013 The Japan Society of Applied Physics
Contact Information